VERDOT InPlace™ column, the best technology for packing & unpacking of Ceramic Hydroxyapatite (CHT)

Ceramic Hydroxyapatite requires special consideration during process scale chromatography packing. This is primarily due to the high specific gravity and rapid settling rate of CHT, moreover for the CHT 80µm that has a free-settling velocity of 125-275 cm/hr.  Additionally, as the CHT media is sensitive to mechanical shear, it can lead to fracturing of particles and creation of fines.
VERDOT InPlace columns offer a unique solution for media such as CHT with smooth slurry transfer, packing in axial compression and unpacking with minimal amount of buffer.


VERDOT expands its range of FlexiPro with two « very low flowrate » disposable fluidic paths

The VERDOT Chromatography skid is now available in its «  very low flowrate » version for  small scale purification processes

FlexiPro ™ chromatography equipment is a compact system with disposable fluid path capable of handling small and medium scale GMP biopharmaceutical molecule purification processes. It has been specifically designed for single-use processes as well as for multi-product production units.

So far the initial range has been offering two fluidic path scales:
  • the “low flow kit” (LFK) that runs from 5 to 150 L / h and
  • the “high flow kit” (HFK) that runs from 20 to 600 L / h.
To meet the growing demand of very small volumes applications (like in the case of orphan diseases, clinical trials, …), VERDOT has developed two new fluid paths compatible with the same flexipro system allowing to work from 0.6 to 7LPH, with Ultra Low Flow kit “(ULFK) and 1 to 30 LPH with the” Very Low Flow Kit “(VLFK).
An adaptation device has been installed enabling the FlexiPro to adapt to the size of each fluid path. As a result the same FlexiPro system can cover wide scale ranges to provide purification of therapeutic molecules ranging from a few grams to several kilograms.

Time to changing the adaptation set and the fluidic path is less than half an hour.

All of the FlexiPro fluidic paths meet the following specifications:
  • 4 sizes of fluidic paths:
    • ULFK: 0.6 – 7LPH (10-116mL / min)
    • VLFK: 1 – 30LPH (17-500mL / min)
    • LFK: 5-120LPH (83-2000mL / min)
    • HFK: 20-600LPH
  • 7 process inputs, spread over 2 pumps
  • Automated inline gradient and dilution possibility
  • High precision disposable instrumentation
  • Possibility of up to 4 bar of operating pressure
  • Flow kits assembled in ISO 7 clean room and delivered in double packaging
  • Documentation and records respecting all regulatory requirements


The new VERDOT Chromatography Column 2S for food, cosmetic and nutraceutical purification

Food, cosmetic and nutraceutical applications often require separation of molecules or families of molecules with high purity, usually from vegetal or milk sources. Process chromatography is a good means for this purification when co-products and target molecules are close to the same size, especially using reverse phase or ion exchange techniques.

Very often, these applications involve food grade media, with large particles that are often subject to swelling and require high Height/Diameter ratio.
Current columns available on the market, with tank-based designs and large distribution chambers, are adequate for product treatment (demineralization, decoloration, debittering,…), but less suitable for molecule purification due to the large dilution induced by their distributors.

The VERDOT Chromatography Column 2S, has been designed from the wheels up for molecule separation applications .

The features of the Verdot Column 2S include:
  • High performance distributors with low void volume for maximum purified product concentration
  • High H/D, customizable for specific applications
  • Various solutions for media replacement, depending on frequency
  • Complete documentation for regulatory submissions
  • Optional configurations with higher solvent resistance
  • Robust design compatible with frequent wash downs in production environments


A versatile system for Continuous Chromatography Purification

Continuous purification processes are emerging as tools to deliver greater productivity and manufacturing efficiency than batch process.  For pilot and production scale continuous purification methods, VERDOT Ips² is pleased to propose its new Multi-Purpose Continuous Purification Platform, the Verdot P3C system.
The Verdot P3C system offers a wide range of capabilities:
  • Simultaneous use of up to 5 chromatography columns with diameters of 1.5 cm to 10.0 cm.
  • Flow ranges of 5 ml/min. to 280 ml/min, with tubing diameters of 0.75 mm to 2.5 mm ID.
  • Operating pressure up to 3.8 bar.
  • Six pumps heads, two flow meters, and in-line continuous pH, Conductivity and UV absorbance sensors.
  • Open design for quick re-configuration of flow pattern.
Customized solutions are available to meet unique requirements.

The features of the control system for the Verdot P3C include:
  • Real-time monitoring of the separation process by continuous flow rate, UV absorbance, Conductivity, and pH measurements.
  • Process run history including all events, alarms, set points and operating sequences.
  • Opportunity for integration with plant-wide information systems through the Open Platform Communication (OPC) server of the P3C system.
  • Straight-forward programming of process sequences using functional blocks.
This platform makes it possible to operate a sequential chromatography scheme in Bind & Elute mode with multiple columns, and to use columns in series with different ligands for performing two purification steps at one time. The isocratic mode, like Simulated Moving Bed, and other continuous purification technique are also possible.

Several applications for the purification of proteins and heterosides have been successfully developed on this platform, and scaled to our larger production systems.


Verdot Ips2 ‘s AgiChrom, a simple solution when LPLC chromatography with packed bed is difficult (Diatomaceous earth, silica, etc.)

Some chromatography supports based on diatomaceous earth, silica, and alumina are rarely used in packed bed chromatography for LPLC applications.    These supports can be difficult to clean, pack consistently, and run at low back pressures. The column packing is tricky due to the physical properties of the particles:  brittle tensile strength limits compressibility and small and variable particle sizes lead to dense packing and high back pressure.
The VERDOT Ips² AgiChrom agitated column circumvents these limitations and allows the user to perform several unit operations in one piece of equipment:
  • Dry Chromatography media can be loaded through the open top. Slurried media can be poured in from the top or pumped through the slurry valves mounted in the bottom flange.
  • The impeller, with motorized height adjustment, allows progressive slurrying of the media in a mobile phase, minimizing the shear stress on chromatography media.
  • The level of liquid in the column can be automatically controlled, by introduction of liquid through the ports on the cover and draining through the bottom filter.
  • The chromatography media can therefore be cleaned in place one or more times. The  mobile phase burdened with particles can be siphoned through a dip tube.
    Then the chromatography media can be mixed with the raw product and the various mobile phases for the mass transfer.
  • At last, the chromatography media can be removed from the column through the slurry valve(s), with automatic column tilting.
The mass transfer in agitated mode allows using of the full media binding capacity, while being relieved from the flow resistance due to chromatography media and product viscosity.
For the same reasons, the AgiChrom column is also very adapted for applications using very soft media unstable in settled bed mode (dextran matrix,…)


MiniPro system as pilot scale LPLC process chromatography system

VERDOT Ips² MiniPro system is now also proposed as pilot scale LPLC process chromatography system.

The VERDOT Ips² MiniPro system, the most compact LPLC process chromatography system in the market, is now also proposed with a low flow configuration (1-60L/h), for serving as pilot chromatography system.

This system can thus be supplied with pilot flow kit (1-60L/h) and process flow kit (5-120L/h) for allowing scale-up with minimal investment. As standard, it is proposed with dual pumps for precise in-line dilution and gradient and it encloses all the instrumentation for fully automated LPLC purification, with safety controls and trends records. One hour presentation suffice for a new User to run the system and program simple or complex purification method.


Packing & unpacking of Sepharose(™)Fast Flow(GE) in InPlace(™) column

Testing was performed in order to evaluate the packing and unpacking capabilities of the Verdot Ips² InPlace column, using Sepharose(™)(1) media.  A 14cm bed of GE Zinc Imac Sepharose(™) Fast Flow media was packed in an 80 cm diameter Verdot Ips² InPlace column, using a compression factor of 1.15.

(1)Sepharose is a Trade mark of GE HEALTHCARE BIO-SCIENCES
Two packings were performed. The initial packing was performed with an empty column, the second following the performance of re-slurrying within the column.  Both packings were evaluated using HETP and Asymmetry tests and provided great results and reproducibility.

Prior to removal, air sparging and the InPlace columns tilting feature were used to re-suspended the media within the column in less than 20 minutes.  Through the column slurry valves, all media was transferred into the slurry tank in less than 5 minutes.