Chromatography LPLC (= Low Pressure Liquid Chromatography) refers to a process working at low pressure (<6bar) allowing a transfer of molecules between:

  • A solid “stationary” phase, often called chromatography “media” or “gel”, chosen to interact with the product to purify
  • A liquid “mobile” phase which refers to the product to purify or any chemical solution which conditions the stationary phase to adsorb or desorb the molecules.

The stationary phase is contained in a chromatography column between 2 distributors which contains the stationary phase and distribute evenly the mobile phase through the entire column surface and transfer it in depth from top to bottom.

LPLC is typically used in the following cases:

  • Fine separation of “high” value molecules ( >2$/g in order of magnitude)….
  • Separating molecules of similar size, where filtration can’t work
  • Fragile molecules, incompatible with precipitation or high velocity (shear stress)
  • Purification and concentration, in Bind & Elute mode: valuable molecules can be very diluted in the load solution and end up contained in the bed volume after adsorption. They can be eluted in »2-5 column volumes.
  • The change of solvent in Bind & Elute mode can help further purify. Example in Reverse Phase Chromatography: load raw product in water, elute the target molecules in EtOH that can be easily evaporated
  • Adaptable to any kind of molecules: nucleic acids (DNA), proteines, glyco-proteins, sugars, Lipids,…

 

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